Worksheet Chapter 1: Introduction to Biology and Laboratory Rules

1. A student investigates the effect of pH on the rate of salivary amylase enzyme activity. Identify the following variables:
(a) Manipulated variable
(b) Responding variable
(c) One fixed variable

2. True or False: On a line graph, the manipulated variable is plotted on the horizontal X-axis.

3. Explain why biological drawings must not contain shading or color fills.

4. True or False: Used culture plates (petri dishes with agar) belong to Category A waste and should be placed in sharp waste containers.

5. An experimental animal carcass (rat) was used for an organ system dissection. Outline the correct steps to dispose of this biological waste according to standard laboratory protocols.

6. Compare and contrast a Fume Hood and a Biological Safety Cabinet (BSC) in terms of their primary functions and what they protect.

7. Which of the following items is classified under Category A biological waste?

8. Describe the proper procedure for managing and disposing of non-sharp soft biological waste (Category B), such as used agar plates and contaminated gloves.

9. Fill in the blank: The study of microorganisms such as bacteria, microscopic fungi, and viruses is called ________.

10. State the function of a Safety Shower in a biology laboratory.